《植物生理学报》 2009, 45(4): 379-384

供锌水平对寒地春玉米幼苗抗氧化酶活性等生理特性的影响

杨克军^1,2,*, 蔡鑫鑫², 张树远², 张崎峰², 王玉凤², 王庆祥¹

1 沈阳农业大学农学院, 沈阳110161; 2 黑龙江八一农垦大学农学院, 黑龙江大庆163319

通信作者：杨克军；E-mail: byndykj@163.com；Tel: 0459-6819179

摘　要：

本试验选用不同耐锌玉米品种, 采用营养液水培, 研究不同锌浓度对玉米株高、地上部干重、根干重及 SOD、POD、 TTC、硝酸还原酶活性、细胞膜透性的影响。结果表明: 耐锌品种 ‘ 牡单九 ’ 的适宜锌浓度为 0.1 µmol•L-1, 锌敏感品种 ‘ 四 单十九 ’ 的适宜锌浓度为 1 µmol•L-1。不同锌浓度对玉米生长的影响主要表现在地上部, 而对地下部生长影响较小。随着 锌浓度的升高, SOD 和 POD 的活性增大。当锌浓度为 0.1 µmol•L-1 时根系活力最高, 在锌浓度大于 1 µmol•L-1 时, 根系活力 下降较明显。硝酸还原酶活性在锌浓度大于0.01 µmol•L-1 时, 随着锌浓度升高而降低。在锌浓度为1 µmol•L-1 时, 细胞膜透 性最小, 低锌和高锌胁迫下细胞膜透性都有不同程度的增加。

关键词：玉米幼苗; 锌水平; 保护酶活性; 硝酸还原酶

收稿：2009-01-21   修定：2009-03-09

资助：国家“十一五” 科技支撑计划项目(2006BAD02A11)和黑龙江省“十一五” 科技攻关项目(GA07B101)。

Effects of Zn on the Antioxidant Enzymes Activities and Physiological Characteristics in Spring Maize

YANG Ke-Jun^1.2,*, CAI Xin-Xin², ZHANG Shu-Yuan², ZHANG Qi-Feng², WANG Yu-Feng²,WANG Qing-Xiang¹

¹College of Agronomy, Shenyang Agricultural University, Shenyang 110161, China; ²College of Agronomy, Heilongjiang August First Land Reclamation University, Daqing, Heilongjiang 163319, China

Corresponding author: YANG Ke-Jun1.; E-mail: byndykj@163.com; Tel: 0459-6819179

Abstract:

In order to investigate the effects of different zinc concentrations on physiological characteristics in maize, different Zn-tolerant maize cultivars were used as materials. Plant height, dry weights of root and shoot, root activity, cell membrane permeability, and activities of SOD, POD, TTC and NR were measured. The results showed that the optimal concentration of Zn in maize cultivar ‘Mudan 9’ and ‘Sidan 19’ were 0.1 µmol·L^-1 and 1 µmol·L^-1, respectively. Different Zn concentrations had more effects on aerial part than underground part. The activities of SOD and POD increased with the increasing of Zn concentration. The Zn concentration of the highest root activity was 0.1 µmol·L^-1, and root activity decreased obviously when Zn concentration was more than 1 µmol·L^-1. When Zn concentration was more than 0.01 µmol·L^-1, NR activity decreased with the increasing of Zn concentration. When Zn concentration was 1 µmol·L^-1, cell membrane permeability was lowest. Cell membrane permeability increased under low or high Zn stress.

Key words: maize seedlings; Zn supply level; protective enzymes activities; NR